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Wednesday, August 12, 2020 | History

8 edition of DNA-protein interactions found in the catalog.

DNA-protein interactions

principles and protocols

  • 193 Want to read
  • 26 Currently reading

Published by Humana Press in Totowa, N.J .
Written in English

    Subjects:
  • DNA-protein interactions

  • Edition Notes

    Includes bibliographical references and index

    Statementedited by Tom Moss
    SeriesMethods in molecular biology -- 148, Methods in molecular biology (Clifton, N.J.) -- 148
    ContributionsMoss, Tom
    Classifications
    LC ClassificationsQP624.75.P74 D57 2001
    The Physical Object
    Paginationxvii, 638 p. :
    Number of Pages638
    ID Numbers
    Open LibraryOL17008103M
    ISBN 100896036251, 0896036715
    LC Control Number00054100

    How to Cite. Ouwerkerk, P. B. and Meijer, A. H. Yeast One-Hybrid Screening for DNA-Protein Interactions. Current Protocols in Molecular Biology. Assay of Restriction Endonucleases Using Oligonucleotides 33 Analysis of DNA-Protein Interactions by Intrinsic Fluorescence, 34 Circular Dichroism for the Analysis of Protein-DNA Interactions, 35 Calorimetry of protein-DNA complexes and their components, 36 Surface plasmon resonance applied to DNA-protein complexes, 37 Reconstitution of Protein.

    The monograph approaches the study of protein-nucleic acid interactions in two distinctive ways. First, DNA-protein and RNA-protein interactions are presented together. Second, the first half of the book develops the principles of protein-nucleic acid recognition, whereas the second half applies these to more specialized topics.   The clear and easy-to-follow protocols collected here illuminate the molecular basis of protein nucleic acid interactions. Use them successfully to reveal the location of the DNA binding site, the strength and specificity of a binding, the identities of individual groups on the actual bases involved in binding, and the specific amino acid residues of the protein that Author: G. Geoff Kneale.

    The mechanism and function of these DNA-protein interactions are presently being investigated, and a number of important discoveries relating to how the helicase proteins interact with DNA have been reported recently. While some of the protein-DNA interactions directly relate to the unwinding function of the enzyme complex, other protein-DNA. HUMANA PRESS HUMANA PRESS Edited by Tom Moss Methods in Molecular Biology TM Methods in Molecular Biology TM VOLUME DNA–Protein Interactions SECOND EDITION SECOND EDITION Principles and Protocols Principles and Protocols Edited by Tom Moss DNA–Protein Interactions POLII TFIIH M e t h o d s i n M o l e c u l a r B I O L O G Y TM John .


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DNA-protein interactions Download PDF EPUB FB2

In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of DNA-protein interactions book interactions and their manner of function, both in the cell and in the test tube.

DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved.

This book presents a concise overview of current research on the biophysics of DNA-protein interactions. A wide range of new and classical methods are presented by authors investigating physical mechanisms by which proteins interact with DNA.

DNA–protein interactions play key roles in the function of regulatory proteins. Because they recognize a sequence of 8–20 bp in a background of millions of base pairs, they are sequence-specific DNA-binding proteins (Travers, ()).

The target sites in DNA are located at strategically important places near promoters. DNA-binding proteins are proteins that have DNA-binding domains and thus have a specific or general affinity for single- or double-stranded DNA.

Sequence-specific DNA-binding proteins generally interact with the major groove of B-DNA, because it exposes more functional groups that identify a base r, there are some known minor groove DNA-binding ligands.

DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry.

In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA. Adhya, in Encyclopedia of Genetics, DNA Binding Properties. DNA–protein interactions play key roles in the function of regulatory proteins.

Because they recognize a sequence of 8–20 bp in a background of millions of base pairs, they are sequence-specific DNA-binding proteins (Travers, ()).The target sites in DNA are located at strategically important places near.

that are the focus of this book: structural, thermodynamic, and bioinformatic. The earliest studies used biochemical and biophysical methods to analyze the thermo-dynamic and kinetic aspects of protein–DNA interactions. The first binding site sequences were determined in the early s, which led to hypotheses about.

Dna-Protein Interactions by Travers, A. and a great selection of related books, art and collectibles available now at   DNA-Protein Interactions: Principles and Protocols, Third Edition (Methods in Molecular Biology) Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation.

Get this from a library. DNA-Protein Interactions. [Tom Moss] -- Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein DNA interactions and define their components, their manner of interaction, and their manner of.

from book Real-Time Analysis of Biomolecular Interactions: Applications of BIACORE (pp) DNA-Protein Interactions Chapter January with 17 Reads. Buy Dna-Protein Interactions Books online at best prices in India by Malcolm Buckle,Andrew Travers from Buy Dna-Protein Interactions online of India’s Largest Online Book Store, Only Genuine Products.

Lowest price and Replacement Guarantee. Cash On Delivery Available. ISBN: OCLC Number: Description: xxii, pages: illustrations ; 25 cm. Contents: Expression systems / Reinhard Grisshammer --Gel electrophoresis and bending in cisplatin-modified linear DNA / Jean-Marc Malinge --Use of DNA microcircles in protein-DNA binding studies / Dominique Payet --Use of.

DNA–protein interactions are fundamental to the existence of life forms, providing the key to the genetic plan as well as mechanisms for its mainte-nance and evolution. The study of these interactions is therefore fundamental to our understanding of growth, development, differentiation, evolution, and Size: 2MB.

"The book, written by an impressive list of worldwide contributors, will provide readers with nearly all the necessary tools for studying DNA-protein interactions, making it a Price: $ There are mainly two broad types of DNA protein interactions: 1) Sequence specific DNA binding and 2) Sequence non-specific DNA binding.

In case of sequence specific DNA protein interactions, a DNA binding protein binds to a DNA on a site having a Cited by: 1. The subject of the book will be the biophysics of DNA-protein interactions, and will include new methods and results that describe the physical mechanism by which proteins interact with DNA.

For example, there has been much recent work on the mechanism by which proteins search for specific binding sites on : Springer New York. This book would focus on the specific topic of the biophysics of DNA-protein interactions, and would include the use of new approaches, including both bulk methods as well as single molecule methods.

This would make the book attractive to anyone working in the general area of DNA-protein interactions, which is of course a much wider market than.

DNA Structure and Function, a timely and comprehensive resource, is intended for any student or scientist interested in DNA structure and its biological implications. The book provides a simple yet comprehensive introduction to nearly all aspects of DNA structure.

It also explains current ideas on the biological significance of classic and alternative DNA s: 1. Nègre N, Cavalli G. Mapping Protein/DNA Interactions by Cross-Linking. Review Identification and mapping of protein-protein interactions by a combination of cross-linking, cleavage, and proteomics.Stanford Libraries' official online search tool for books, media, journals, databases, government documents and more.Univ.

Laval, Quebec, Canada. Offers an array of more than current and reproducible techniques biochemists and molecular, cellular, and developmental biologists can use to illuminate DNA-protein interactions.

Includes protocols for in vivo footprinting. Hardcover, softcover not available.